Exploration of genetic variability in Garcinia [Garcinia gummi-gutta L.(Robson)] germplasm based on growth, yield and quality traits
DOI:
https://doi.org/10.24154/jhs.v19i1.2628Keywords:
Correlation analysis, DNA fingerprints, garcinia, principal component analysisAbstract
Eleven Garcinia germplasm along with local check of Pechiparai were evaluated and underwent principal component analysis to assess genetic divergence and variation patterns during 2019 to 2022. The first two principal components, contributing significant Eigen values, explained 71.20% of the total variability. The Acc. Gg 9 was the top performer, exhibiting favourable yield and growth traits with lower pest and disease incidence, high biochemical compounds viz., hydroxy citric acid and tartaric acid compared to local check. Cluster analysis revealed four major clusters, offering diversity for breeding programs. Correlation studies highlighted traits such as number of fruits per tree, rind thickness, and tartaric acid showing significant positive correlations with yield per tree. Selection based on identified key traits was deemed crucial for enhancing effectiveness. Additionally, DNA fingerprinting analysis indicated the potential use of RAPD markers (OPA03570) for differentiating Kudampuli cultivar PPI (K) 1 from the local check. Overall, the present investigation provides insights into optimizing Garcinia breeding programs, emphasizing trait-based selection and DNA fingerprinting for varietal differentiation.
References
Angami, T., Wangchu, L., Debnath, P., Sarma, P., Singh, B., Singh, A. K., Singh, S., Hazarika, B. N., Singh, M. C., Aochen, C. & Lungmuana (2021). Garcinia L.: a gold mine of future therapeutics. Genetic Resources and Crop Evolution, 68, 11-24.
Aysen Melda Colak & Melda Colak A. (2000). Morphological and biochemical diversity in fruits of Arbutus unedo L. from east Aegean region in Turkey. Erwerbsobstbau, 61, 379-383.
Chen, P. Y., Yu, J. W., Lu, F. L., Lin, M. C. and Cheng, H. F. (2016). Differentiating parts of Cinnamomum cassia using LC q-tof MS in conjunction with principal component analysis. Biomedical Chromatography, 30(9), 1449-1457.
Gawel, N. J. & Jarret R. L. (1991). A modified CTAB DNA extraction procedure for Musa and Ipomoea plant. Molecular Biology Reports, 9, 262-266.
Hemshekhar, M., Sunitha, K., Santhosh, M. S., Devaraja, S., Kemparaju, K., Vishwanath, B.S., Niranjana, S. R. & Girish, K. S. (2011). An overview on genus Garcinia: phytochemical and therapeutical aspects. Phytochemistry Reviews, 10, 325-351.
Hossain, M. B., Patras, A., Barry-Ryan, C., Martin-Diana, A. B. and Brunton, N. P. (2011). Application of principal component and hierarchical cluster analysis to classify different spices based on in vitro antioxidant activity and individual polyphenolic antioxidant compounds. Journal of Functional Foods, 3(3), 179-189.
Liyanagamage, D. S. N. K., Jayasinghe, S., Priyadarshani Attanayake, A. & Karunaratne, V. (2020). Correlation between antioxidant activity and the garcinol content released from fruit rinds of endemic Garcinia quaesita Pierre on different cooking conditions. Journal of Food Quality, 2020, 1-7.
Mathew, G., Mathew, B., Shaneeb, M. and Nyanthara, B. (2011a). Diuretic activity of leaves of Garcinia cambogia in rats. Indian Journal of Pharmaceutical Sciences, 73(1), 228-230.
Mathew G. E., Mathew B., Sheneeb, M. M., Nyanthara & Haribabu Y. (2011b). Anthelmintic activity of leaves of Garcinia cambogia. International Journal of Research in Pharmaceutical Sciences, 2(1), 63-65.
Machamangalath, R., Arekar, C. & Lele, S. S. (2016). Exotic tropical fruit wines from Garcinia indica and Musa acuminate. Journal of the Institute of Brewing, 122(4), 745-753.
Parthasarathy, Utpala & Nandakishore, O. P. (2014). Morphological characterisation of some important Indian Garcinia species. Dataset Papers in Science, 1-4.
Priya Devi, S., Balamohan, T. N., Thangam, M., Ashokkumar, J., Ramachananduu, K., & Krorikanthimath (2012). A study on diversity and distribution of kokum (Garcinia indica (Choisy) Thouars) using DIVA-GIS in Goa with respect to fruit charecters. Indian Journal of Horticulture, 69(2), 156-162.
Rencher, Alvin, C. & Christensen, W. F. (2002). Methods of multivariate analysis. A John Wiley & Son. Inc. Publication, 727.
Roopa, G. S. & Kasiviswanatham, V. (2013). Extraction of tartaric acid from tamarind pulp and analysis of the acid composition in leaves. International Journal of Students’ Research in Technology & Management, 1(5), 478-488.
Satish, M. & Misra-Thakur, S. (2008). Estimation of hydroxycitric acid (-)-hca in Garcinia indica choisy by HPLC method. Analytical Chemistry: An Indian Journal, 7(12), 854-856.
Singh, P., Roy, T. K., Kanupriya, C., Tripathi, P.C., Kumar, P. & Shivashankara, K. S., (2022). Evaluation of bioactive constituents of Garcinia indica (kokum) as a potential source of hydroxycitric acid, anthocyanin, and phenolic compounds. LWT, 156, 112999.
Sivalingam, P., Singh, D. & Chauhan, S. (2012). Morphological and molecular diversity of an underutilized fruit crop - Cordiamyxa L. germplasm from the arid region of Rajasthan, India. Genetic Resources and Crop Evolution,59, 10.1007/s10722-011-9781-7.
Shivakumar, S., Sandhiya, S., Subhasree, N., Agrawal, A. & Dubey, G. P. (2013). In vitro assessment of antibacterial and antioxidant activities of fruit rind extracts of Garcinia cambogia. L. International Journal of Pharmacy and Pharmaceutical Sciences, 5(2), 254-257.
Shara, M., Ohia, S. E., Schmidt, R. E., Yasmin, T., Zardetto Smith A., Kincaid, A., et al. (2004). Physico-chemical properties of a novel (-)- hydroxycitric acid extract and its effect on body weight, selected organ weights, hepatic lipid peroxidation and DNA fragmentation, hematology and clinical chemistry, and histopathological changes over a period of 90 days. Molecular and Cellular Biochemistry, 260, 171-186.
Utpala, P., Asish, G. R., Jayarajan, K., Aravind, R., Krishnamoorthy, B. & Mathew, P. A. (2010). Isozyme diversity of Garcinia gummigutta (L.) N. Robson in Western Ghat region, South India. Journal of Spices and Aromatic Crops, 19(1/2), 29-33.
Wang, Z., Sun, F., Xie, S., Wang, J., Li, Y., Dong, J., Sun, M. & Sun, B., (2019). A new species of Garcinia (Clusiaceae) from the middle Miocene of Fujian, China, and a phytogeographic analysis. Geological Journal, 54(3), 1317-1330.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 S.T. Bini Sundar Sundar, Ashokkumar G, Jaya Jasmine A; Vasanth S
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.