Molecular and biological detection of impatiens necrotic spot virus (INSV) isolate from ornamental plants in Iran
DOI:
https://doi.org/10.24154/jhs.v18i2.1827Keywords:
DAS-ELISA, INSV, Ornamental viruses, RT-PCRAbstract
Impatiens necrotic spot virus (INSV), belong to genus Orthotospovirus, causes severe damage to greenhouse ornamental plants. INSV reported from almost every ornamental screen house in Iran. INSV general symptoms include necrotic leaf spot, chlorosis and stunting in infected ornamental host plants. A total number of 581 ornamental samples of 58 different plant species with symptoms similar to those of INSV infection were collected in 4 provinces of Iran. The results indicated an average of 20.13 per cent virus incidence. INSV infection was recorded to be 28% in Mahallat, 24.5% in Tehran, 22.1% in Mazandaran and 16.4% in Guilan provinces. No record of INSV infection on ornamental samples was obtained from Khouzistan province (Dezful). From the total of 58 ornamental species tested, 33 species recorded positive for INSV. ELISA positive samples were rechecked by RT-PCR using a set of specific primers directed to the N-gene region, which were designed to detect and characterize the virus species. The primers ampl fied a 777 bp product of the nucleoprotein as shown by agarose gel electrophoresis. The nucleotide sequence of amplicons was compared with related sequences, using Blast software available at NCBI GenBank, which showed highest similarity with impatiens necrotic spot virus (INSV) isolates. Accordingly, all genome components of the isolate shared 89-96.5 % nucleotide sequence identities with corresponding sequence of other Iranian and GenBank INSV isolates. The Iranian isolates were all placed in the same group and bore the most similarity to INSV isolates from the Netherlands, Italy and United States. In phylogenetic analysis based on the partial nucleotide and deduced amino acid sequences of N region, INSV from Iran appears to be closely related to the Japanese AB1099100 Verbena spp. isolate.
References
Anonymous. (2018). Department of statistics. Ministry of agriculture. Agricultural statistics of Iran. Tehran. Iran. Volume 2.
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389-3402. doi: 10.1093/nar/25.17.3389
Amruta, B. S., Laxmidevi, V., Ramegowda, G. K., Seetharamu, G. K., Usharani, R. T., & Krishnareddy, M. K. (2020). First report of Groundnut bud necrosis virus infecting anthurium (Anthurium andreanum) in India. New Disease Reporter, 41, 14. doi.org/10.5197/j.2044-0588.2020.041.014
Bananej, K., Shahraeen, N., Ahoonmanesh, A., Lesemann, D. E., & Shahreyare, D. (1998). Identification of tomato spotted wilt virus from tomato fields in Varamin. Iranian Journal of Plant Pathology, 34, 18-26.
Bayat, H., & Nazerian, E. (2019). Introduction of new hosts for the genus Orthotospovirus from Iran. First plant pathology congress of Iran, Karaj. August, 21-23. p.191.
Broadbent, A. B., & Allen, W. R. (1995). Interactions within the western flower thrips tomato spotted wilt virus, host plant complex on virus epidemiology. Thrips Biology and Management, 185-196. doi: 10.1007/978-1-4899-1409-5_27
Clark, M. F., & Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34, 475-485. doi: 10.1099/0022-1317-34-3-475.
Chung, B. N., Pak, H. S., Jung, J. A., & Kim, J. S. (2006). Occurrence of tomato spotted wilt virus in Chrysanthemum (Dendranthema grandiflorum) in Korea. Plant Pathology Journal, 22(33), 230-134. doi.org/10.5423/PPJ.2006.22.3.230
Daughtrey, M. L., Jones, R. K., Moyer, J. W., Daub, M. E., & Baker, J. R. (1997). Tospoviruses strike the greenhouse industry, INSV has become a major pathogen on flower crops. Plant Disease, 81(11), 1220-1229.doi: 10.1094/PDIS.1997.81.11.1220
EPPO. (1999). Impatiens necrotic spot tospovirus. EPPO Bulletin, 29, 473-476. doi.org/10.1111/j.1365-2338. 1999.tb00801.x
German, T. L., Ullman, D. E., & Moyer, J. W. (1992). Tospoviruses: diagnosis, molecular biology, phylogeny and vector relationships. Annal Review of Phytopathology, 30, 315-48. doi.org/10/1146/annurev.py.30.090192.001531
Ghotbi, T., & Shahraeen, N. (2022). Diagnosis of important virus diseases of ornamental Anthurium in screen-houses in Tehran province. International Journal of Medical & Pharmaceutical Science, 12(5), 1-6. doi: http://dx.doi.org/10.31782/IJMPS.2022.12501
Ghotbi, T., & Nazerian, E. (2010). Report on incidence of Cucumber mosaic virus (CMV) on ornamental Anthurium in Iran. 19th Plant Protection Congress, Tehran, Iran. 31 July, 3 August, 2010. p. 722.
Ghotbi, T., Gillasian, E., & Shahraeen, N. (2003). Detection of tospoviruses in individual thrips by ELISA from ornamental plants in Tehran and Markazi provinces. Iranian Journal of Applied Entomology and Phytopathology, 70(2), 138-139.
Ghotbi, T., & Shahraeen, N. (2012). Incidence and distribution of viruses infecting propagated ornamentals in Northern Iran. International Research Journal of Microbiology, 3(11), 373-381. http://www.interesjournals.org/IRJM
Ghotbi, T., Shahraeen, N., & Winter, S. (2005). Occurrence of tospoviruses in ornamental and weed species in Markazi and Tehran provinces in Iran. Plant Disease, 89(4), 425-429. https://doi.org/10.1094/PD-89-0425
Golnaraghi, A. R., Shahraeen, N., Pourrahim, R., Ghorbani, Sh., & Farzadfar, Sh. (2001a). First report of a Tospovirus infection of peanuts in Iran. Plant Disease, 85(12), 1286. doi: 10.1094/PDIS.2001.85.12.1286C
Mehraban, A. H., & Shahraeen, N. (2000). Identification of tomato spotted wilt virus (TSWV) from Ornamentals in Mahallat area. 14th Iranian Plant Protection Congress, Sept. 2000, Isfahan, Iran.
Naidu, R. A., Doem, C. M., & Sherwood, J. L. (2005). Expansion of the host range of impatiens necrotic spot virus to peppers. Online. Plant Health Progress, doi: 10.1094/PHP-2005-0727-01-HN
Peters, D. (1998). An updated list of plant species susceptible to tospoviruses. Fourth International Symposium on Tospovirus and Thrips in Floral and Vegetable Crops, 2-6 May, Wageningen, pp. 107-110.
Poelwijk, F., Prince, M., & Golbakh, R. (1997). Completion of the impatiens necrotic spot virus genome sequence and genetic comparison of the L proteins within the family Bunyaviridae. Journal of General Virology, 78, 543-546. doi: 10.1099/0022-1317-78-3-543
Pourrahim, R., Golnaraghi, A., & Farzadfar, Sh. (2012). Occurrence of impatiens necrotic spot virus and tomato spotted wilt virus from Iran. Plant Disease, 96(5), 771-771. https://doi.org/10.1094/PDIS-01-12-0051-PDN
Shahraeen, N., & Bananaj, K. (1996). Occurrence of peanut mottle virus in Gorgan Province. Iranian Journal of Plant Pathology, 32(1-2). p. 21.
Shahraeen, N., Ghotbi, T., & Mehraban, A. H. (2002). Occurrence of impatiens necrotic spot virus in ornamentals in Mahallat and Tehran provinces in Iran. Plant Disease, 86(6), 694. https://doi.org/10.1094/PDIS.2002.86.6.694A
Shahraeen, N., & Ghotbi, T. (2003). Natural occurrence of different tospovirus species infecting ornamentals and other agricultural crops in Iran. International Congress of Plant Pathology, ICCP 2-7 February.
Nei, M., & Kumar, S. (2000). Molecular evolution and phylogenetics. Oxford University Press. New York. p. 219.
Tammara, K., Stecher, G., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetic analysis version 6.0. Molecular Biology Evolution, 30, 2725-2729. doi: 10.1093/molbev/mst197
Zavareh, N., Ghotbi, T., & Maleki, M. (2013). Detection and diagnosis of cucumber mosaic virus from Anthurium main commercial cultivars in Tehran province. Iranian Congress of Virology, Tarbiyat Modares University, Tehran, Iran, 18-20, October 2013.
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