Studies on Genetic Divergence in Pomegranate (Punica granatum L.) Using SRAP Markers
DOI:
https://doi.org/10.24154/jhs.v10i2.116Keywords:
Pomegranate, Molecular Markers, SRAP, Genetic DiversityAbstract
Pomegranate genotypes have been characterized mainly on the basis of morphological traits; but, these traits are affected to a large extent by environmental and cultivation conditions, resulting in their ambiguous discrimination. Molecular markers are more suited for accurate discrimination of genotypes and cultivars. Sequence-Related Amplified Polymorphism (SRAP) markers were used in the present study to analyze polymorphism among the important pomegranate genotypes grown in India. The total number of bands generated by 30 SRAP primers for 12 genotypes was 1448, with an average of 48.3 bands per primer. Polymorphism varied from 2.7 to 73.9, with an average of 40.95%. Similarity-value based on Jaccard's Coefficient ranged from 0.63 (between cvs. Naina and Amlidana) to 0.95 (between cvs. Kabul Yellow and Jalore Seedless). UPGMA (un-weighted pair group method with arithmetic mean) analysis was performed and a dendrogram was constructed using Jaccard's similarity matrix. The 12 genotypes used grouped into 5 clusters. SRAP markers were found suitable for determining variability among the pomegranate genotypes studied.
References
Aradhya, M. 2006. http://www.ars-grin.gov/npgs/cgc_reports/woody2006/ncgrdavis2006.html
Budak, H., Shearman, R.C., Parmaksiz, I., Gaussoin, R.E., Riordan T.P. and Dweikat, I. 2004. Molecular characterization of buffalo grass germplasm using sequence-related amplified polymorphism markers. Theor. Appl. Genet., 108:328-334
Ebrahimi, S., Sayed Tabatabaei, B.E. and Sharifnabi, B. 2010. Microsatellite isolation and characterization in pomegranate (Punica granatum L.). Iranian J. Biotech., 8:156-163
Ferriol, M., Pico, B. and Nuez, F. 2003. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP Markers. Theor. Appl. Genet., 107:271-282
Gulsen, O., Karagul, S. and Abak, K. 2007. Diversity and relationships among Turkish okra germplasm by
SRAP and phenotypic marker polymorphism. Biologia Bratislava, 62:41-45
Hasnaoui, N., Mars, M., Chibani, J. and Trifi, M. 2010. Molecular polymorphism in Tunisian pomegranate (Punica granatum L.) as revealed by RAPD fingerprints. Diversity, 2:107-113
Jbir, R., Hasnaoui, N., Mars, M., Marrakchi, M. and Trifi, M. 2008. Characterization of Tunisian pomegranate (Punica granatum L.) cultivars using amplified fragment length polymorphism analysis. Sci. Hortic., 115:231-237
Kanupriya, C., Nischita, P. and Ravishankar, K.V. 2013. An efficient method of genomic DNA isolation from pomegranate. Indian J. Hort., 70:584-586
Kumar, L.S. 1999. DNA markers in plant improvement. Biotech. Adv., 17:143-183
Li, G. and Quiros, C.F. 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 103:455-461
Moslemi, M., Zahravi, M. and Bakhshi Khaniki, G. 2010. Genetic diversity and population genetic structure of pomegranate (Punica granatum L.) in Iran using AFLP markers. Sci. Hortic., 126:441-447
Narzary, D., Rana, T.S. and Ranade, S.A. 2010. Genetic diversity in inter-simple sequence repeat profiles across natural populations of Indian pomegranate (Punica granatum L.). Pl. Biol., 12:806-813
Parvaresh, M., Talebi, M., Ebrahim, B. and Tabatabaei, S. 2012. Molecular diversity and genetic relationship of pomegranate (Punica granatum L.) genotypes using microsatellite markers. Sci. Hortic., 138:244-252
Pirseyedi, S.M., Valizadehghan, S., Mardi, M., Ghaffari, M.R., Mahmoodi, M., Zahravi, M., Zeinalabedini, M.
and Khayam Nekoui, S.M. 2010. Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.). Int’l. J. Mol. Sci., 11:2010-2016
Rohlf, F.J. 2000. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.2. Exeter Software. Setauket, New York, USA
Sarkhosh, A., Zamani, Z., Fatahi, R. and Ranjbar, H. 2009. Evaluation of genetic diversity among Iranian soft- seed pomegranate accessions by fruit characteristics and RAPD markers. Sci. Hortic., 121:313-319
Shishodia, S., Adams, L., Bhatt, I.D. and Aggarwal, B.B. 2006. Anticancer potential of pomegranate. In: Pomegranates. Ancient Roots to Modern Medicine, 1st edn. Taylor and Francis Group, Boca Raton, FL, USA, pp. 107-116
Yuan, H., Yin, Y., Qu, J., Zhu, L. and Li, Y. 2007. Population genetic diversity in Chinese pomegranate (Punica granatum L.) cultivars revealed by fluorescent-AFLP markers. J. Genet. Genom., 34:1061-1071
Downloads
Published
Issue
Section
License
Copyright (c) 2015 C Kanupriya, D Manmohan Kumar, P Nischita, M Gayathri, K V Ravishankar, P Sampath Kumar (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
How to Cite
. 
